Direct evidence that RNA inhibits APOBEC3G ssDNA cytidine deaminase activity
نویسندگان
چکیده
منابع مشابه
The double-domain cytidine deaminase APOBEC3G is a cellular site-specific RNA editing enzyme
APOBEC3G is a cytidine deaminase with two homologous domains and restricts retroelements and HIV-1. APOBEC3G deaminates single-stranded DNAs via its C-terminal domain, whereas the N-terminal domain is considered non-catalytic. Although APOBEC3G is known to bind RNAs, APOBEC3G-mediated RNA editing has not been observed. We recently discovered RNA editing by the single-domain enzyme APOBEC3A in i...
متن کامل7SL RNA mediates virion packaging of the antiviral cytidine deaminase APOBEC3G.
Cytidine deaminase APOBEC3G (A3G) has broad antiviral activity against diverse retroviruses and/or retrotransposons, and its antiviral functions are believed to rely on its encapsidation into virions in an RNA-dependent fashion. However, the cofactors of A3G virion packaging have not yet been identified. We demonstrate here that A3G selectively interacts with certain polymerase III (Pol III)-de...
متن کاملAntiviral Function of APOBEC3G Can Be Dissociated from Cytidine Deaminase Activity
The antiretroviral activity of the cellular enzyme APOBEC3G has been attributed to the excessive deamination of cytidine (C) to uridine (U) in minus strand reverse transcripts, a process resulting in guanosine (G) to adenosine (A) hypermutation of plus strand DNAs. The HIV-1 Vif protein counteracts APOBEC3G by inducing proteasomal degradation and exclusion from virions through recruitment of a ...
متن کاملLYMPHOID NEOPLASIA APOBEC3G enhances lymphoma cell radioresistance by promoting cytidine deaminase-dependent DNA repair
1Department of Pathology and the Lautenberg Center for General and Tumor Immunology, Hebrew University–Hadassah Medical School, Jerusalem, Israel; 2Institute of Chemistry and Center for Nanoscience and Nanotechnology, Hebrew University, Jerusalem, Israel; 3Department of Genetics, Institute of Life Sciences, Hebrew University, Jerusalem, Israel; 4Division of Hematology and Bone Marrow Transplant...
متن کاملAPOBEC3G enhances lymphoma cell radioresistance by promoting cytidine deaminase-dependent DNA repair.
APOBEC3 proteins catalyze deamination of cytidines in single-stranded DNA (ssDNA), providing innate protection against retroviral replication by inducing deleterious dC > dU hypermutation of replication intermediates. APOBEC3G expression is induced in mitogen-activated lymphocytes; however, no physiologic role related to lymphoid cell proliferation has yet to be determined. Moreover, whether AP...
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ژورنال
عنوان ژورنال: Biochemical and Biophysical Research Communications
سال: 2011
ISSN: 0006-291X
DOI: 10.1016/j.bbrc.2011.08.009